Publications

2022

Liu D*, Thélot FA*, Piccirilli JA, Liao M^#, Yin P^#. Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly. Nature Methods. 2022 May 2. doi:10.1038/s41592-022-01455-w. Epub ahead of print. PMID: 35501384. *Co-first author. ^#Co-corresponding author.

- Wyss Institute News: Nanotechnology enables visualization of RNA structures at near-atomic resolution

Arlt H, Sui X, Folger B, Adams C, Chen X, Remme R, Hamprecht FA, DiMaio F, Liao M, Goodman JM, Farese RV Jr, Walther TC. Seipin forms a flexible cage at lipid droplet formation sites. Nat Struct Mol Biol. 2022 Mar;29(3):194-202. doi: 10.1038/s41594-021-00718-y. Epub 2022 Feb 24. PMID: 35210614; PMCID: PMC8930772.
2021

Baez-Nieto D, Allen A, Akers-Campbell S, Yang L, Budnik N, Pupo A, Shin YC, Genovese G, Liao M, Pérez-Palma E, Heyne H, Lal D, Lipscombe D, Pan JQ. Analysing an allelic series of rare missense variants of CACNA1I in a Swedish schizophrenia cohort. Brain. 2021 Dec 17:awab443. doi: 10.1093/brain/awab443. Epub ahead of print. PMID: 34919654.

Thélot FA, Zhang W, Song K, Xu C, Huang J, Liao M. Distinct allosteric mechanisms of first-generation MsbA inhibitors. Science. 2021 Oct 29;374(6567):580-585. doi: 10.1126/science.abi9009. Epub 2021 Sep 23. PMID: 34554829.

- HMS News: Combating Antibiotic Resistance (also on Medical Express)

- Featured in Current Biology: How to avoid a post-antibiotic age (by Michael Gross)

Sharma S*, Zhou R*, Wan L, Shan Feng, Song K, Xu C, Li Y^#, Liao M^#. Mechanism of LolCDE as a molecular extruder of bacterial triacylated lipoproteins. Nat Commun 12, 4687 (2021). doi: 10.1038/s41467-021-24965-1. *Co-first author. ^#Co-corresponding author.

Plummer AM*, Culbertson AT*, Liao M. The ABCs of Sterol Transport. Annu Rev Physiol. 2021 Feb 10;83:153-181. doi: 10.1146/annurev-physiol-031620-094944. Epub 2020 Nov 3. PMID: 33141631. *Co-first author.
2020

Luo M*, Zhou W*, Patel H, Srivastava AP, Symersky J, Bonar MM, Faraldo-Gómez JD^#, Liao M^#, Mueller DM^#. Bedaquiline inhibits the yeast and human mitochondrial ATP synthases. Commun Biol. 2020 Aug 19;3(1):452. doi: 10.1038/s42003-020-01173-z. PMID: 32814813; PMCID: PMC7438494. *Co-first author; #Co-corresponding author.

Fan M, Zhang J, Tsai CW, Orlando BJ, Rodriguez M, Xu Y, Liao M, Tsai MF, Feng L. Structure and mechanism of the mitochondrial Ca²⁺ uniporter holocomplex. Nature. 2020 Jun;582(7810):129-133. doi: 10.1038/s41586-020-2309-6. Epub 2020 May 20. PMID: 32494073.

Sui X, Wang K, Gluchowski NL, Elliott SD, Liao M^#, Walther TC^#, Farese RV Jr^#. Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature. 2020 May;581(7808):323-328. doi: 10.1038/s41586-020-2289-6. Epub 2020 May 13. PMID: 32433611. ^#Co-corresponding author.

Orlando BJ, Liao M. ABCG2 transports anticancer drugs via a closed-to-open switch. Nat Commun. 2020 May 8;11(1):2264. doi: 10.1038/s41467-020-16155-2. PMID: 32385283; PMCID: PMC7210939.
- Selected for Editors' Highlights (https://www.nature.com/collections/hhfigaahch)

Thélot F, Orlando BJ, Li Y, Liao M. High-resolution views of lipopolysaccharide translocation driven by ABC transporters MsbA and LptB2FGC. Curr Opin Struct Biol. 2020 Apr 23;63:26-33. doi: 10.1016/j.sbi.2020.03.005. Epub ahead of print. PMID: 32335504.

Wu X, Siggel M, Ovchinnikov S, Mi W, Svetlov V, Nudler E, Liao M, Hummer G, Rapoport TA. Structural basis of ER-associated protein degradation mediated by the Hrd1 ubiquitin ligase complex. Science. 2020 Apr 24;368(6489):eaaz2449. doi: 10.1126/science.aaz2449. PMID: 32327568.

2015 - 2019
Structure and mechanism of the cation-chloride cotransporter NKCC1.
Chew TA*, Orlando BJ*, Zhang J*, Latorraca NR, Wang A, Hollingsworth SA, Chen DH, Dror RO, Liao M^#, Feng L^#.

Nature. 2019 Aug;572(7770):488-492. doi: 10.1038/s41586-019-1438-2.

PMID: 31367042

EMDB (PDB): 0470 (6NPH), 0471 (6NPJ), 0472 (6NPK), 0473 (6NPL), 0474, 0475
Large protein organelles form a new iron sequestration system with high storage capacity.
Giessen TW^#, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M^#, Silver PA^#.

Elife. 2019 Jul 8;8. pii: e46070. doi: 10.7554/eLife.46070.

PMID: 31282860; PMCID: PMC6668986

EMDB (PDB): 9383 (6NJ8)
PDB: 6N63
Structural basis of lipopolysaccharide extraction by the LptB2FGC complex.
Li Y*, Orlando BJ*, Liao M.

Nature. 2019 Mar;567(7749):486-490. doi:10.1038/s41586-019-1025-6.

PMID: 30894744; PMCID: PMC6532066

EMDB: 9118 (LptB2FG), 9125 (LptB2FGC, final map), 9128 (LptB2FGC, map with clear LPS density), 9129 (LptB2FGC, βJRlong map), 9130 (LptB2FGC, βJRshort map), 9124 (vanadate-trapped LptB2FG) and 9126 (vanadate-trapped LptB2FGC).
PDB: 6MHU (LptB2FG), 6MI7 (LptB2FGC), 6MHZ (vanadate-trapped LptB2FG) and 6MI8 (vanadate-trapped LptB2FGC)
Structural basis of coreceptor recognition by HIV-1 envelope spike.
Shaik MM, Peng H, Lu J, Rits-Volloch S, Xu C, Liao M, Chen B.

Nature. 2019 Jan;565(7739):318-323. doi: 10.1038/s41586-018-0804-9.

PMID: 30542158; PMCID: PMC6391877.
DNA melting initiates the RAG catalytic pathway.
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M^#, Wu H^#.

Nat Struct Mol Biol. 2018 Aug;25(8):732-742. doi:10.1038/s41594-018-0098-5.

PMID: 30061602; PMCID: PMC6080600
Cryo-electron microscopy structure of the lipid droplet-formation protein seipin.
Sui X, Arlt H, Brock KP, Lai ZW, DiMaio F, Marks DS, Liao M^#, Farese RV Jr^#,
Walther TC^#.

J Cell Biol. 2018 Oct 16. pii: jcb.201809067. doi:10.1083/jcb.201809067.

PMID: 30327422
EMDB: 9146
PDB: 6MLU
X-ray and cryo-EM structures of the mitochondrial calcium uniporter.
Fan C*, Fan M*, Orlando BJ*, Fastman NM*, Zhang J, Xu Y, Chambers MG, Xu X, Perry
K, Liao M^#, Feng L^#.

Nature. 2018 Jul 11. doi: 10.1038/s41586-018-0330-9.
View full article: https://rdcu.be/2QLu

PMID: 29995856
EMDB: 7800(Ma in A8–35) | 7801(Fg in PMAL-C8) | 7802,7803,7804(Fg in nanodiscs: all,type1,type2)
PDB: 6C5W | 6C5R
Structure basis for RNA-guided DNA degradation by Cascade and Cas3.
Xiao Y*, Luo M*, Dolan AE, Liao M^# , Ke A^#.

Science. 2018 Jul 6;361(6397). pii: eaat0839. doi: 10.1126/science.aat0839.

PMID: 29880725
EMDB: 7347 (prenicking state) | 7346 (postnicking state)
PDB: 6C66 (prenicking state)
High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane.
Srivastava AP*, Luo M*, Zhou W, Symersky J, Bai D, Chambers MG, Faraldo-Gómez JD, Liao M^#, Mueller DM^#.

Science. 2018 Apr 12. pii: eaas9699. doi:10.1126/science.aas9699
Abstract | Reprint | Full text

PMID: 29650704
EMDB: 7548(F1Fo) | 7549(Fo) | 7546(F1Fo-oligomycin) | 7547(Fo-oligomycin)
PDB: 6CP6(F1Fo) | 6CP7(Fo) | 6CP3(F1Fo-oligomycin) | 6CP5(Fo-oligomycin)
Structural basis of MsbA-mediated lipopolysaccharide transport.
Wei Mi, Yanyan Li, Sung Hwan Yoon, Robert K. Ernst, Thomas Walz, Maofu Liao.

Nature. 2017 Sep 6. doi: 10.1038/nature23649.
View full article: http://rdcu.be/vCR7

PMID: 28869968
EMDB: 8469 | 8467 | 8465 | 8669 | 8670 | 8671 PDB: 5TV4 | 5TTP
Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.
Stefan Schoebel*, Wei Mi*, Alexander Stein, Sergey Ovchinnikov, Ryan Pavlovicz, Frank DiMaio, David Baker, Melissa G. Chambers, Huayou Su, Dongsheng Li, Tom A. Rapoport^#, Maofu Liao^#.

Nature. 2017 Aug 17;548(7667):352-355. doi: 10.1038/nature23314.

PMID: 28682307
EMDB: 8637 | 8642 | 8639 | 8638 PDB: 5V6P | 5V7V EM Data: EMPIAR-10099
Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.
Yibei Xiao*, Min Luo*, Robert P. Hayes, Jonathan Kim, Sherwin Ng, Fang Ding, Maofu Liao^#, Ailong Ke^#.

Cell. 2017 Jun 29;170(1):48-60.e11. doi: 10.1016/j.cell.2017.06.012..

PMID: 28666122
EMDB: 8478 | 8477 PDB: 5U0A | 5U07

Read more @ HMS News, Science Newsline, Phys.org, and Science Daily.
Molecular mechanism of V(D)J recombination from synaptic RAG1-RAG2 complex structures.
Heng Ru, Melissa G. Chambers, Tian-Min Fu, Alexander B. Tong, Maofu Liao^#, Hao Wu^#.

Cell. 2015 Nov 19;163(5):1138-52. doi: 10.1016/j.cell.2015.10.055.

PMID: 26548953 PMCID: PMC4690471
EMDB: 6487 | 6488 | 6489 | 6490 | 6491 PDB: 3JBW | 3JBX | 3JBY EM Data: EMPIAR-10049
2010 - 2014

Publications at UCSF (Molecular Electron Microscopy)

Gira Bhabha, Hui-Chun Cheng, Nan Zhang, Arne Moeller, Maofu Liao, Jeffrey A. Speir, Yifan Cheng, Ronald D. Vale. (2014) Allosteric communication in the dynein motor domain. Cell 159:857-68. PMID: 25417161

Maofu Liao, Erhu Cao, David Julius and Yifan Cheng. (2014) Single particle electron cryo-microscopy of a mammalian ion channel. Curr Opin Struct Biol. 27: 1–7. PMID: 24681231

Maofu Liao*, Erhu Cao*, David Julius and Yifan Cheng. (2013) Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature 504, 107–112. (* equal contribution) PMID: 24305160
(See also NEWS & VIEWS by Richard Henderson, UCSF research story)

Erhu Cao*, Maofu Liao*, Yifan Cheng and David Julius. (2013) TRPV1 structures in distinct conformations reveal activation mechanisms. Nature 504, 113–118. (* equal contribution) PMID: 24305161

Daniele Canzio, Maofu Liao, Nariman Naber, Ed Pate, Adam Larson, Shenping Wu, Diana Marina, Jennifer Garcia, Hiten Madhani, Roger Cooke, Peter Schuck, Yifan Cheng, Geeta J. Narlikar. (2013) A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly. Nature 496: 377–381. PMID: 23485968

Dina Schneidman-Duhovny, Andrea Rossi, Agustin Avila-Sakar, Seung Joong Kim, Javier Velázquez-Muriel, Pavel Strop, Hong Liang, Kristin A. Krukenberg, Maofu Liao, Ho Min Kim, Solmaz Sobhanifar, Volker Dötsch, Arvind Rajpal, Jaume Pons, David A. Agard, Yifan Cheng and Andrej Sali. (2012) A method for integrative structure determination of protein-protein complexes. Bioinformatics 28(24): 3282-3289. PMID: 23093611

Shenping Wu, Agustin Avila-Sakar, JungMin Kim, David S. Booth, Charles H. Greenberg, Andrea Rossi, Maofu Liao, Xueming Li, Akram Alian, Sarah L. Griner, Narinobu Juge, Yadong Yu, Claudia M. Mergel, Javier Chaparro-Riggers, Pavel Strop, Robert Tampé, Robert H. Edwards, Robert M. Stroud, Charles S. Craik, Yifan Cheng. (2012) Fabs enable single particle cryoEM studies of small proteins. Structure 20(4): 582-92. PMID: 22483106

Metka Lenassi, Gerard Cagney, Maofu Liao, Tomaž Vaupotič, Koen Bartholomeeusen, Yifan Cheng, Nevan J. Krogan, Ana Plemenitaš, B. Matija Peterlin. (2010) HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells. Traffic 11: 110-122. PMID: 19912576
2004 - 2010

Publications at Albert Einstein College of Medicine (Protein Biochemistry and Virus Membrane Fusion)

Margaret Kielian, Chantal Chanel-Vos and Maofu Liao. (2010) Alphavirus entry and membrane fusion. Viruses 2010, 2(4): 796-825. PMID: 21546978

Maofu Liao, Claudia Sánchez-San Martín, Aihua Zheng and Margaret Kielian. (2010) In vitro reconstitution reveals key intermediate states of trimer formation by the dengue virus membrane fusion protein. Journal of Virology 84(11): 5730-40. PMID: 20335260

M. Umashankar, Claudia Sánchez-San Martín, Maofu Liao, Brigid Reilly, Alice Guo, Gwen Taylor, and Margaret Kielian. (2008) Differential cholesterol binding by class II fusion proteins determines membrane fusion properties. Journal of Virology 82(18): 9245-53. PMID: 18632857

Maofu Liao and Margaret Kielian. (2006) Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly. Journal of Virology 80(22): 11362-11369. PMID: 16971447

Maofu Liao and Margaret Kielian. (2006). Site-directed antibodies against the stem region reveal low pH-induced conformational changes of the Semliki Forest virus fusion protein. Journal of Virology 80(19): 9599-9607. PMID: 16973563

Maofu Liao and Margaret Kielian. (2005). Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion. Journal of Cell Biology 171(1): 111-120. PMID: 16216925

Maofu Liao and Margaret Kielian. (2005). The conserved glycine residues in the transmembrane domain of the Semliki Forest virus fusion protein are not required for assembly and fusion. Virology 332(1): 430-437. PMID: 15661173

Don L. Gibbons, Anna Ahn, Maofu Liao, Lena Hammar, R. Holland Cheng, and Margaret Kielian. (2004) Multistep regulation of membrane insertion of the fusion peptide of Semliki Forest virus. Journal of Virology 78(7): 3312-3318. PMID: 15016852
2000

Publications at Tsinghua University (HIV vaccine design)

Maofu Liao, Yun Lu, Yi Xiao, Manfred P. Dierich, Ying-Hua Chen. (2000) Induction of high level of specific antibody response to the neutralizing epitope ELDKWA on HIV-1 gp41 by peptide-vaccine. Peptides. 21(4): 463-468. PMID: 10822100

Yi Xiao, Maofu Liao, Yun Lu, Manfred P. Dierich, Ying-Hua Chen. (2000) Epitope-vaccines: a new strategy to induce high levels of neutralizing antibodies against HIV-1. Immunobiology 201(3-4): 323-331. PMID: 10776789

Publications

2022

2021

2020

2015 - 2019

2010 - 2014

Publications at UCSF (Molecular Electron Microscopy)

2004 - 2010

Publications at Albert Einstein College of Medicine (Protein Biochemistry and Virus Membrane Fusion)

2000

Publications at Tsinghua University (HIV vaccine design)

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Publications

2022

2021

2020

2015 - 2019

2010 - 2014

Publications at UCSF (Molecular Electron Microscopy)

2004 - 2010

Publications at Albert Einstein College of Medicine (Protein Biochemistry and Virus Membrane Fusion)

2000

Publications at Tsinghua University (HIV vaccine design)